Ultra Performance Liquid Chromatography (UPLC)
Chemical Analysis
Purity Analysis using Ultra-Performance Liquid Chromatography (UPLC) is a high-resolution analytical technique used to determine the purity of pharmaceutical compounds, chemicals, natural products, food additives, and research samples. UPLC provides rapid, precise, and highly sensitive separation of target compounds and impurities, making it an essential tool for quality control, research, and product development. Ultra-Performance Liquid Chromatography (UPLC) separates compounds based on their chemical properties using high-pressure liquid chromatography with sub-2 μm particle columns. Compared with conventional HPLC, UPLC offers superior resolution, higher sensitivity, shorter analysis time, and lower solvent consumption. Purity Analysis by UPLC identifies the principal compound and detects impurities or degradation products within the sample. The technique is widely used in pharmaceutical quality control, food safety, biotechnology, environmental analysis, and academic research.
Test specification
- Methodology
- Ultra-Performance Liquid Chromatography (UPLC) - Reverse Phase Chromatography (RP-UPLC) (where applicable) - Gradient or Isocratic Elution - UV/Vis, PDA, or Fluorescence Detection (depending on method) - Quantitative Chromatographic Analysis
- Standards followed
Testing is performed according to internationally recognized standards where applicable, including: - USP (United States Pharmacopeia) - EP (European Pharmacopoeia) - BP (British Pharmacopoeia) - ICH Q2(R2) – Validation of Analytical Procedures - AOAC Official Methods (where applicable) - ISO/IEC 17025 Laboratory Practices
- Equipment used
- Ultra-Performance Liquid Chromatography (UPLC) System - Binary Solvent Manager - Sample Manager (Autosampler) - Column Manager - UV/Vis or PDA Detector - Chromatography Data System (CDS)
- Parameters measured
- Compound Purity (%) - Retention Time - Peak Area - Peak Height - Impurity Level - Relative Retention Time (RRT) - Resolution - Tailing Factor - Theoretical Plates - Concentration - Chromatographic Profile
- Measurement range
| Parameter | Typical Range | |--|--| | Purity | 0–100% | | Concentration | μg/mL to mg/mL | | Injection Volume | 1–10 μL | | Analysis Time | 2–20 minutes (Method dependent) | | Detection Wavelength | 190–800 nm (Detector dependent) |
- Accuracy / detection limit
### Accuracy - Quantitative Accuracy: Typically **±1–2%** - Excellent repeatability and reproducibility under validated methods. ### Detection Limit - Limit of Detection (LOD): **Low ppb to ppm level** (compound dependent) - Limit of Quantification (LOQ): Determined according to validated analytical methods.
Suitability
- Applications
- - Pharmaceutical Quality Control - Drug Purity Testing - Chemical Purity Analysis - Impurity Profiling - Stability Studies - Natural Product Analysis - Food Additive Analysis - Nutraceutical Analysis - Cosmetic Ingredient Analysis - Research & Development - Method Development - Process Validation
- Industries served
- - Pharmaceutical Industries - Biotechnology Companies - Universities & Research Institutions - Food & Beverage Industry - Chemical Manufacturing - Cosmetics Industry - Nutraceutical Industry - Environmental Laboratories - Contract Research Organizations (CROs) - Healthcare Laboratories
- Advantages
- High resolution and excellent peak separation. - Faster analysis than conventional HPLC. - High sensitivity for trace impurities. - Low solvent consumption. - Excellent reproducibility. - Suitable for pharmaceutical and research applications. - Accurate quantitative analysis. - Internationally accepted analytical technique.
- Limitations
- Samples must be soluble in suitable solvents. - Method development may be required for unknown compounds. - Matrix interference may affect separation. - Requires high-purity solvents and analytical-grade reagents. - Not suitable for highly particulate samples without proper filtration.
Submitting a sample
- Sample requirement
- Samples should be completely dissolved in a suitable solvent. - Solutions should be filtered through a **0.22 μm or 0.45 μm membrane filter** before analysis. - Sample concentration should be appropriate for the analytical method. - Typical injection volume: **1–10 μL**. - Recommended sample volume: **2–10 mL** for liquid samples. - Samples must be free from particulate matter.
- Turnaround time
| Service | Estimated Time | |-|-| | Standard Analysis | 3–7 Working Days | | Priority Service | 24–48 Hours (Subject to availability) |
- Deliverables
Clients will receive: - Official UPLC Test Report - Chromatogram - Peak Integration Table - Purity Percentage - Impurity Profile (where applicable) - Quantitative Results - PDF Report - Optional Raw Data Files
Request this test
Send us your sample details and we'll confirm scope and timing.